目的 研究干旱胁迫下苦豆子子叶中赖氨酸脱羧酶(lysine decarboxylase,LDC)基因表达量与苦参碱(matrine,MA)和氧化苦参碱(oxymatrine,OMA)含量的关系。方法 以不同质量分数PEG 6000胁迫萌动苦豆子种子,72 h后高效液相色谱(HPLC)测定其苦参碱和氧化苦参碱含量,荧光定量PCR(Real-time fluorescence quantitative PCR,qPCR)测定赖氨酸脱羧酶表达量。结果 萌动苦豆子种子在轻度胁迫(PEG质量分数＜15%)下,子叶中苦参碱和氧化苦参碱含量降低,重度胁迫(PEG质量分数＞20%)下,子叶中苦参碱和氧化苦参碱含量升高。荧光定量PCR显示,子叶中赖氨酸脱羧酶表达量随胁迫加剧呈先降后升的趋势,特别是在轻度和重度胁迫下,赖氨酸脱羧酶表达量与苦参碱和氧化苦参碱含量变化一致。结论 苦豆子子叶中苦参碱和氧化苦参碱含量与赖氨酸脱羧酶的表达有一定的关系。
Abstract
OBJECTIVE To study the effect of lysine decarboxylase (LDC) gene on the accumulation of matrine(MA) and oxymatrine (OMA) in cotyledon of Sophora alopecuroides L. germinating seeds. METHODS The S.alopecuroides germinating seeds were stressed by different mass fractions of PEG 6000, and the contents of MA and OMA were determined by high performance liquid chromatography (HPLC) and the expression level of LDC was analyzed by real-time fluorescence quantitative PCR (qPCR) after 72 h treatment. RESULTS The contents of MA and OMA decreased in the cotyledon under light stress (PEG mass fraction<15%), while increased with the stress getting higher (PEG mass fraction>20%). The analysis of qPCR revealed that the LDC expression level was decreased first, and then increased with the stress rising. The changes of the contents of MA and OMA were parallel with the expression level of LDC especially under light and severe stress. CONCLUSION There is certain association between the accumulation of MA and OMA and the gene expression quantity of LDC. The results is of significance for illustrating the role of LDC in the biosynthetic pathways of MA and OMA.
关键词
苦豆子 /
氧化苦参碱 /
赖氨酸脱羧酶基因(LDC) /
荧光定量PCR /
基因表达
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Key words
Sophora alopecuroides L. /
oxymatrine /
lysine decarboxylase gene (LDC) /
real-time fluorescence quantitative PCR (qPCR) /
gene expression
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中图分类号：
R917
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脚注
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基金
宁夏回族自治区自然科学基金(NZ14033)
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